Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

Techniques: Activity Assay

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Relative gene expression levels of various mRNAs in the FaDu cells transfected with angiopoietin-like 4 small interfering RNA were determined by reverse transcription-quantitative PCR. The angiopoietin-like 4 level decreased by 38%, and MKI67 expression increased significantly. The expression of BAX decreased, and that of BCL2 increased. CASP3 expression also increased in the angiopoietin-like 4 knockdown cells.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Gene Expression, Transfection, Small Interfering RNA, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Knockdown

Journal: Poultry Science

Article Title: Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens

doi: 10.1016/j.psj.2026.106749

Figure Lengend Snippet: SC suppresses apoptosis in vivo . Ovarian Granulosa Cells intervene in multiple pathophysiological indicators. Examination of apoptosis in laying hens by TUNEL assay. (A-B) Results revealed that treatment with different doses of SC resulted in decreased apoptosis. (C-J) The protein and mRNA expression of Bax, Cyt c, Cleaved Caspase-3, and Bcl-2.

Article Snippet: The membranes underwent blocking with 5% skimmed milk diluted in PBS, and afterwards incubated employing primary antibodies against β-actin (GB15001, Servicebio, Wuhan, China), IP3R (GB11742, Servicebio, Wuhan, China), GRP75 (GB11852, Servicebio, Wuhan, China), VDAC1 ( GB111939 , Servicebio, Wuhan, China), Bcl-2 (26593-1-AP, Proteintech, Wuhan, China), Bax (50599-2-Ig, Proteintech, Wuhan, China), Cyt c (WL02410, Wanleibio, Shenyang, China), Cleaved Caspase-3 (WL01992, Wanleibio, Shenyang, China), GPR41 ( OM184469 , Omnimabs, California, United States), GPR43 ( OM255320 , Omnimabs, California, United States), Occludin (WL01996, Wanleibio, Shenyang, China) and ZO-1(21773-1-AP, Proteintech, Wuhan, China) overnight at 4°C with gentle shaking.

Techniques: In Vivo, TUNEL Assay, Expressing

Journal: Translational Oncology

Article Title: The brain imaging feature-related gene NRP2 drives the malignant progression of glioblastoma through the FAK pathway: a Mendelian randomization study

doi: 10.1016/j.tranon.2026.102732

Figure Lengend Snippet: NRP2 mediates the FAK signaling pathway to regulate the malignant progression of GBM cells. (A): NRP2 expression levels in patients from the GSE4290 and GSE68848 data sets. (B): The knockdown effect of sh-NRP2 in U251 and T98G cells was detected by qRT-PCR. (C): WB was used to detect the expressions of pY297-FAK and FAK. (D): The CCK-8 method was used to detect the cell viability of GBM cells in different treatment groups (sh-NC, sh-NRP2, sh-NRP2 + adhesamine). Adhesamine is a FAK activator. (E): The colony formation experiment was used to detect the number of colony formations of GBM cells in different treatment groups; (F): The scratch healing experiment was used to detect the migration ability of GBM cells in different treatment groups; (G): The Transwell experiment was used to detect the invasion ability of GBM cells in different treatment groups; (H): The Annexin V/PI double staining method was used to detect the apoptosis level of cells in different treatment groups. (I): Western blot was used to detect the protein expression of pro-apoptotic indicators clever-caspase3, clever-PARP, BAX and pro-apoptotic indicator BCL2 in different treatment groups. * indicates P < 0.05, **** indicates P < 0.0001.

Article Snippet: BCL2 , Proteintech , China , 12,789-1-AP , Rabbit , 1:9000.

Techniques: Expressing, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Migration, Double Staining, Western Blot

Journal: Translational Oncology

Article Title: The brain imaging feature-related gene NRP2 drives the malignant progression of glioblastoma through the FAK pathway: a Mendelian randomization study

doi: 10.1016/j.tranon.2026.102732

Figure Lengend Snippet: NRP2 promotes the malignant progression of GBM by activating FAK. U251 and T98G cell groups: sh-NC, sh-NRP2, sh-NRP2 + oe-FAK. (A): qRT-PCR was used to detect the mRNA level of FAK; (B): WB was used to detect the protein expression of pY397-FAK and FAK; (C): CCK-8 was used to detect cell viability; (D): Scratch test was used to detect the migration ability of cells; (E): Transwell test was used to detect the invasion ability of cells; (F): WB was used to detect the protein expression of apoptotic proteins clever-caspase3, clever-PARP, BAX and anti-apoptotic protein BCL2. * indicates P < 0.05.

Article Snippet: BCL2 , Proteintech , China , 12,789-1-AP , Rabbit , 1:9000.

Techniques: Quantitative RT-PCR, Expressing, CCK-8 Assay, Migration

Journal: Translational Oncology

Article Title: The brain imaging feature-related gene NRP2 drives the malignant progression of glioblastoma through the FAK pathway: a Mendelian randomization study

doi: 10.1016/j.tranon.2026.102732

Figure Lengend Snippet: The nude mouse experiment demonstrates that NRP2 mediates the Focal-adhesion pathway to regulate the formation of GBM tumors. (A): Nude mice were subcutaneously injected with sh-NRP2 and sh-NC stably transfected T98G cells. Comparison of tumor size in sh-NRP2 nude mice treated with FAK activator adhesamine. (B): Tumor weights of different treatment groups of nude mice. (C): Tumor volumes of different treatment groups of nude mice. (D): IHC detection of the expression of Ki67, BCL2 and BAX in tumor tissues; (E): WB detection of the expression of pY297-FAK and FAK in tumor tissues.

Article Snippet: BCL2 , Proteintech , China , 12,789-1-AP , Rabbit , 1:9000.

Techniques: Injection, Stable Transfection, Transfection, Comparison, Expressing

Journal: bioRxiv

Article Title: Comparison studies between Cesium-137 and X-ray irradiators in epithelial injury using in vitro and in vivo models

doi: 10.64898/2026.04.17.719248

Figure Lengend Snippet: RT-qPCR analysis of CDKN1A ( a, f, k ), KLF5 ( b, g, l ), KLF4 ( c, h, m ), BAX ( d, i, n ), and BCL2 ( e, j, o ) in HCT116, RKO and DLD-1 calculated as a fold change at 6, 24, 48 and 72 h after irradiation. HPRT1 was used as a housekeeping gene (control). RT-qPCR was performed as described in the “Materials and methods” section. Data points represent the average of three independent experiments, with the mean ±SD indicated. Significance was determined by the Student’s test followed by an analysis of the normal distribution (Tukey’s test), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Commercially available TaqMan primers detecting mouse ChgA (Mm00514341-FAM), Alpi1 (Mm01285814-FAM), Olfm4 (Mm01320260-FAM), Cdkn1a (Mm00432448-FAM), Lgr5 (Mm00438890-FAM), Mki67 (Mm01278617-FAM), Actb (Mm04394036-VIC) and human CDKN1A (Hs00355782-FAM), KLF4 (Hs00358836-FAM), KLF5 (Hs0000000-FAM), BCL2 (Hs01048932-FAM), BAX (Hs00180269-FAM) and HPRT1 (Hs02800695-VIC) transcripts were used.

Techniques: Quantitative RT-PCR, Irradiation, Control